منابع مشابه
Polymerase chain reaction
INTRODUCTION On April 25, 1953 James D. Watson and Francis Crick publish "a radically different structure" for DNA, thereby founding the field of Molecular Genetics. Their structure involves two strands of complementary base-paired DNA, running in opposite directions as a double helix. They conclude their report saying that "It has not escaped our notice that the specific pairing we have postul...
متن کاملMicrofluidic polymerase chain reaction
George Maltezos, Alvaro Gomez, Jiang Zhong, Frank A. Gomez, and Axel Scherer Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, MS 200-36 Pasadena, California 91125, USA Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032-8202, USA Keck School of Medi...
متن کاملPolymerase Chain Reaction
s The polymerase chain reaction (PCR) is a technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. There are three major steps in...
متن کاملThe polymerase chain reaction.
This essay on the polymerase chain reaction is one of a series developed as part of FASEB's efforts to educate the general public, and the legislators whom it elects, about the benefits of fundamental biomedical research-particularly how investment in such research leads to scientific progress, improved health, and economic well-being.
متن کاملPolymerase chain displacement reaction.
Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material. We describe a new technique, polymerase chain displacement reaction (PCDR), which uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal q...
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ژورنال
عنوان ژورنال: BMJ
سال: 1997
ISSN: 0959-8138,1468-5833
DOI: 10.1136/bmj.314.7073.5